Clathrin-mediated endocytosis is a highly orchestrated process in which a multitude of proteins partake. These proteins can largely be divided into three groups: the scaffolding protein complex Clathrin, the adaptor complexes (ADAPTOR PROTEIN 2 COMPLEX and the TPLATE COMPLEX) and lastly endocytic accessory proteins (EAPs). EAPs facilitate endocytosis in a plethora of ways. For example, DYNAMIN-RELATED proteins performs fission of mature clathrin-coated pits and AUXILIN-LIKE proteins perform vesicle uncoating, whereas PICALM1 specifically recognize VAMP721 for internalization. However, many EAPs are not described or their effector function is not clear. We are looking for novel endocytic effectors based on a proteomics approach using known endocytic players as bait.  Using a combination of mutant analysis and cell biology we aim to determine the function of the proteins and localize them in the process of clathrin-mediated endocytosis.